|
|
| Year : 2019 | Volume
: 36
| Issue : 1 | Page : 18-21 |
|
| Fine needle aspiration cytology of cysticercosis: A study of 30 cases |
|
|
Subhash Bhardwaj1, Gousia Rather2
1 Department of Pathology, Govt. Medical College, Jammu, Jammu and Kashmir, India
2 Department of Pathology, Govt. Medical College, Srinagar, Jammu and Kashmir, India
Click here for correspondence address and email
| Date of Web Publication | 17-Jan-2019 |
|
|
 |
|
 Abstract | | |
Cysticercosis is a systemic parasitic disease caused by larval stage of Taenia solium. It is the most common parasitic disease worldwide. It is potentially a dangerous systemic disease with variable clinical manifestations. It can be diagnosed by radiological imaging and serology but demonstration of parasitic larva by cytology or histopathology is still the gold standard. Fine needle aspiration cytology (FNAC) plays an important role in prompt diagnosis of this disease. Aim: To study the role of FNAC in the diagnosis of cysticercosis. Materials and Methods: This study included 30 patients presenting with palpable subcutaneous and intramuscular nodules at different sites. Fine needle aspiration was performed on these lesions and cytological features were analyzed. Subsequent excision biopsy was evaluated wherever possible. Results: Thirty patients in the age group of 7–80 years presenting with subcutaneous and intramuscular nodules at various sites were studied. Majority of the patients were males. The most commonly affected sites were upper and lower extremities and abdominal wall. Most of the cases were clinically misdiagnosed as lipomas, neurofibromas, lymphadenitis, epidermal inclusion cyst, sialadenitis, and soft tissue tumors. On cytological examination of aspirate from the lesions, fragments of parasite were seen in a background of mixed inflammatory cell infiltrate. Histopathological correlation was available in 17 cases. Conclusion: FNAC is a simple, sensitive, cost-effective, and rapid diagnostic tool for diagnosis of cysticercosis as cytological diagnosis is quite clear where the actual parasitic structures are identified in the smear.
Keywords: Cysticercosis, fine needle aspiration cytology, parasite
How to cite this article:
Bhardwaj S, Rather G. Fine needle aspiration cytology of cysticercosis: A study of 30 cases. J Cytol 2019;36:18-21 |
| Introduction | |  |
Cysticercosis, caused by larval stage of pork tapeworm Taenia solium, is the most common parasitic disease worldwide with an estimated prevalence of more than 50 million persons. It is endemic in Mexico, Central and South America, parts of Africa, Asia, and India.[1] In India, it is more common in northern parts.[2] Human cysticercosis commonly manifests as subcutaneous or intramuscular nodules, which may clinically be mistaken for commoner lesions such as lipoma, inclusion cysts, and lymphadenopathies. Since cysticercosis presents mostly as palpable swelling, the lesion is easily amenable for FNAC, which is a rapid and minimally invasive investigation to diagnose this condition.[3]
FNAC is a simple, sensitive, cost-effective, and rapid diagnostic tool for evaluation of any superficial palpable lump over various sites of the body.[4] The diagnostic role of FNAC in cysticercosis was first emphasized by Kung et al. in 1989.[5] Since then, FNAC has become a pivotal tool in evaluating subcutaneous and muscle nodules caused by parasites.[6] A spectrum of cytologic details of cysticercosis covering the entire range from viable cysts through necrotic and calcified lesions has been described.[7]
This study illustrates the role of FNAC in definitive diagnosis of cysticercosis, even in clinically unsuspected cases.
| Materials and Methods | | |
This study was carried out in the cytology section of our department. The study included 30 patients presenting with palpable subcutaneous and intramuscular nodules at different sites. Age and sex of the patient, site and size of the lesion, and clinical diagnosis were noted.
Fine needle aspiration was performed with a 23G needle and a 20 ml disposable syringe. The nature of aspirated material was noted and it was smeared on the glass slides. In case of fluid aspirated from cystic lesions, smears were prepared from cyst fluid after cytocentrifugation. The slides were stained with MGG stain after air drying and with Papanicolaou stain after fixation with 95% ethyl alcohol. Subsequent excision biopsy was evaluated wherever possible. The histopathological findings were correlated with cytological findings.
| Results | | |
This study included 30 patients in the age group of 7–80 years. Majority of patients (30%) were in the age group of 21–30 years [Table 1]; 18 patients (60%) were males and 12 (40%) were females. The most common affected site was upper extremities (26.66% cases) followed by lower extremities and abdominal wall (16.66% cases each). Other affected sites were chest wall, back, neck, tip of tongue, and axilla [Table 2]. Two patients presented with multiple nodules. On local examination, swellings were soft to firm, nontender, and nodular and ranged in size from 1 to 4.5 cm. Provisional diagnosis of parasitic lesion was made in only one case, whereas the majority was clinically misdiagnosed as lipomas, neurofibromas, lymphadenitis, epidermal inclusion cysts, sialadenitis, and soft tissue tumors [Table 3]. Aspirated material in maximum number of cases consisted of clear fluid with granular deposits. In five cases, aspirate was purulent and in three cases it was hemorrhagic.
Microscopic examination of the smears revealed numerous fragments of the parasitic bladder wall in all cases. These appeared as granular, loose fibrillary sheath-like structures. The fragments had multiple subcuticular cells with small blue pyknotic nuclei [Figure 1]a and [Figure 1]b. The background of the smears was dirty eosinophilic granular in 21 cases, clean in five cases and necrotic in four cases. Varying degrees of mixed inflammatory cell infiltrate were seen, which comprised eosinophils (21 cases), lymphocytes (23 cases), neutrophils (14 cases), and histiocytes, including palisading histiocytes (20 cases). Multinucleated foreign body type giant cells were seen in 13 cases. Fibroblasts were also seen in seven cases. None of the cases showed hooklets or scolices. | Figure 1: (a) Fragments of fibrillar material (bladder wall) with small blue nuclei (MGG stain x100). (b) Fragments of fibrillar material (bladder wall) with mixed inflammatory infiltrate in the background (Papanicolaou stain x100)
Click here to view |
Histology was available in 17 cases. Larval Taenia solium cysts were seen in the sections in five cases. Lamellated multilayered cyst walls with protoscolices and suckers were identified in these sections. Hooklets were not seen [Figure 2]a. Rest of the cases showed fragments of cyst wall. The cyst wall fragments were multilayered with an outer cuticular layer, middle cellular layer, and an inner fibrillary layer [Figure 2]b. | Figure 2: (a) Larva of cysticercus cellulosae. A protoscolex with paired suckers is identified [hematoxylin and eosin (haematoxylin and eosin x100). (b) Multilayered cyst wall of cysticercus cellulosae (haematoxylin and eosin x400)
Click here to view |
| Discussion | | |
Cysticercosis has been termed as a “biological marker” of the social and economic development of a community.[8] It is a major public health problem, especially in the developing world, being endemic in Mexico, Central and South America, Asia, India, sub-Saharan Africa, and China.[1],[8],[9],[10],[11] Humans acquire this infection by drinking contaminated water or consumption of undercooked pork or raw vegetables, such as cabbage, radish, and carrots that harbor the larva.[12],[13]
The clinical manifestations of the disease depend on location and number of lesions at a particular site.[14] Although the parasite can be found in any organ, they are especially common in central nervous system (CNS), eyes, skeletal muscle, and subcutaneous tissues.[15] All the cases in our study presented with subcutaneous and intramuscular nodules. Upper extremity was the most common site to be affected followed by lower extremity and abdominal wall. There was one case with nodule over the tongue, which is an unusual location for cysticercosis.[16] One case presented with multiple foci, which is also an unusual finding.[17]
The palpable parasitic nodules are often clinically misinterpreted as benign mesenchymal tumors, such as lipoma, neurofibroma, or as lymphadenopathy.[15],[18] In our study too, all except one case were clinically misdiagnosed.
Various diagnostic modalities employed to detect cysticercosis include radiology, serology, and pathological examination. Computed tomography scan and magnetic resonance imaging, though sensitive in diagnosing cysticercosis, especially when the parasite involves the CNS, are very expensive. Serological tests are useful if positive but cannot rule out the disease with negative results. FNAC has emerged as a widely acceptable method for diagnosis of cysticercosis.[15],[18] Aspiration of clear fluid is a strong pointer towards parasitic infestation, although in a significant number of cases aspirate may also be purulent or hemorrhagic.[15],[19] Majority of our cases (20) yielded clear fluid, whereas aspirate was purulent in five cases and hemorrhagic in four cases.
Fully developed cysticerci are opalescent, milky white cysts, oval to elongated, and about 1 cm in diameter. The cyst contains clear fluid and invaginated scolex, which has a rostellum, four suckers, and 22–32 small hooklets. On tissue sections, the cyst wall appears multilayered with an outer cuticular layer, which is smooth, hyalinized, frequently thrown into projections, and covered with microtrichiae. Beneath the tegument is a row of tiny tegumental cells. The inner layer or parenchyma is loose and reticular, containing mesenchymal cells and calcareous corpuscles.[12],[15],[20],[21],[22]
The definitive cytological diagnosis of cysticercosis requires the demonstration of fibrillary sheath/bladder wall, hooklets, and calcareous spherules. However, in the absence of a parasitic wall/sheath, the following subtle morphological indicators give a clue to diagnosis: aspiration of clear watery fluid, dirty, necrotic, granular background in smears, host reaction comprising predominantly of eosinophils or mixed inflammatory infiltrate, palisading histiocytes, scattered plump fibroblasts, or multinucleated giant cells. The predominance of eosinophils in the background provides a vital clue to the parasitic etiology and should prompt the cytologist to search meticulously for a parasite or remnants of parasitic structures such as scattered calcareous spherules, or an occasional hooklet.[3]
The presence of scolex in cytology smears is an unusual finding.[13],[20],[23] In all our cases, we identified only the parenchymal layer with a loose fibrillary stroma. None of our cases showed hooklets/scolices. Goyal et al. have reported similar findings in their study.[24] Histopathological correlation was available in 17 of our cases, which reconfirmed the diagnosis of cysticercosis. In studies by Handa et al.[15] and Goyal et al.,[24] histopathological correlation was available in a small percentage of cases, which proves that FNAC alone can prove useful and cost-effective in the diagnosis of superficial cysticercal lesions.
Subcutaneous and intramuscular palpable parasitic nodule is most commonly due to cysticerci. Other parasites producing similar clinical presentation include Coenuri, the larval form of tapeworm of the genus Multiceps and Spargana, larva of Spirometra mansonoides.[15] Hydatid cyst caused by larva of Echinococcus granulosus can also occur in the subcutaneous tissue as an unusual presentation.[7]
Cytomorphological details of the aspirate help to differentiate these parasites [Table 4]. Cysticerci and Coenuri have suckers and hooklets, whereas Spargana do not. The Coenuri has multiple protoscolices distinguishing it from cysticercus which has a single scolex and is difficult to see in cytology smears. Bladder wall is thin and membranous in cysticerci, whereas it is thicker and lamellated in hydatid cyst. Multiple small scolices in the aspirate obtained from hydatid cyst are in contrast to the single scolex of cysticerci.[15] | Table 4: Distinguishing features of cysticercus cellulosae from other cestodes
Click here to view |
| Conclusion | | |
FNAC is a quick, reliable, and low-cost outpatient procedure for diagnosis of subcutaneous nodules caused by cysticercosis. It is one of the tools for preoperative diagnosis and may even obviate the need for open biopsy and subsequent histopathological examination as the parasite may not be demonstrated even in biopsy specimens.[12],[11],[15] The cytological diagnosis is quite straightforward in cases where the actual parasite structure is identified in the smears. However, in other cases, the presence of eosinophils, histiocytes, and a typical granular dirty background are the features which should always alert the pathologist to this possibility.[12],[17]
In all inflammatory/cystic lesions, particularly in endemic areas, the possibility of cysticercosis should be taken into consideration. An early diagnosis and prompt institution of therapy for superficial cysticercal lesions is essential to prevent dangerous sequelae. A rapid, safe, and reliable cytologic diagnosis of superficial cysticercal lesions by FNAC on an outpatient basis proves to be a cost-effective procedure.
Financial support and sponsorship
Nil.
Conflicts of interest
There are no conflicts of interest.
| References | | |
| 1. | Kraft R. Cysticercosis: An emerging parasitic disease. Am Fam Physician 2007;76:91-6.  |
| 2. | Chatterjee KD. Parasitology, Protozoology and Helminthology in Relation to Clinical Medicine. 12 th ed. Calcutta: Chatterjee Medical Publishers; 1980. p. 116-32. |
| 3. | Pal S, Singh N, Chowdhury N, Huda F, Rao S. Cysticercosis: Reiterating the role of cytodiagnosis. Diagn Cytopathol 2017;45:971-5. |
| 4. | Saran RK, Rattan V, Rajwanshi A, Nijkawan R, Gupta SK. Cysticercosis of the oral cavity: Report of five cases and a review of literature. Int J Paediatr Dent 1998;8:273-8. |
| 5. | Richards F Jr., Schantz PM. Laboratory diagnosis of cysticercosis. Clin Lab Med 1991;11:1011-28. |
| 6. | García HH, Gonzalez AE, Evans CA, Gilman RH, Cysticercosis Working Group in Peru. Taenia solium cysticercosis. Lancet 2003;362:547-56. |
| 7. | Singh N, Arora VK, Bhatia A. Are all subcutaneous parasitic cysts cysticercosis? Acta Cytol 2006;50:114-5. |
| 8. | Prasad KN, Prasad A, Verma A, Singh AK. Human cysticercosis and Indian scenario: A review. J Biosci 2008;33:571-82. |
| 9. | Kamal MM, Grover SV. Cytomorphology of subcutaneous cysticercosis. A report of 10 cases. Acta Cytol 1995;39:809-12. |
| 10. | Naik D, Srinath M, Kumar A. Soft tissue cysticercosis – Ultrasonographic spectrum of the disease. Indian J Radiol Imaging 2011;21:60-2. [ PUBMED] [Full text] |
| 11. | Rao RN, Krishnani N, Malhotra K, Suresh B, Mehrotra R. Dilemmas in cytodiagnosis of subcutaneous swellings: Mimics and look-alikes of cysticercosis. J Clin Pathol 2010;63:926-9. |
| 12. | Gill M, Dua S, Gill P, Gupta V, Gupta S, Sen R, et al. Cytomorphological spectrum of subcutaneous and intramuscular cysticercosis: A study of 22 cases. J Cytol 2010;27:123-6. [ PUBMED] [Full text] |
| 13. | Rajwanshi A, Radhika S, Das A, Jayaram N, Banerjee CK. Fine-needle aspiration cytology in the diagnosis of cysticercosis presenting as palpable nodules. Diagn Cytopathol 1991;7:517-9. |
| 14. | White AC Jr., Weller PF. Harrisons Principles of Internal Medicine. Cestodes. 16 th ed. USA: McGraw-hill; 2005. p. 1272. |
| 15. | Handa U, Garg S, Mohan H. Fine needle aspiration in the diagnosis of subcutaneous cysticercosis. Diagn Cytopathol 2008;36:183-7. |
| 16. | Srivastava P, Kolte S, Gupta K. FNAC a diagnostic modality in cysticercosis: Study of 148 cases with a brief review. Trop J Med Res 2016;19:159-61. [Full text] |
| 17. | Kala P, Khare P. Fine-needle aspiration cytology as a diagnostic modality for cysticercosis: A clinicocytological study of 137 cases. J Cytol 2014;31:68-72. [ PUBMED] [Full text] |
| 18. | Goyal P, Ghosh S, Sehgal S, Mittal D, Singh S. Solitary cysticercosis of parotid gland diagnosed on FNAC. APSP J Case Rep 2014;5:11. |
| 19. | Khurana N, Jain S. Cytomorphological spectrum of cysticercosis – A review of 132 cases. Indian J Pathol Microbiol 1999;42:69-71. [ PUBMED] [Full text] |
| 20. | Verma K, Kapila K. Fine needle aspiration diagnosis of cysticercosis in soft tissue swellings. Acta Cytol 1989;33:663-6. |
| 21. | Arora VK, Gupta K, Singh N, Bhatia A. Cytomorphologic panorama of cysticercosis on fine needle aspiration. A review of 298 cases. Acta Cytol 1994;38:377-80. |
| 22. | Batrani M, Kaushal M, Chaturvedi NK, Yadav R. Fine needle aspiration of subcutaneous cysticercosis. Diagn Cytopathol 2010;38:347-8. |
| 23. | Kumar ND, Misra K. Fine-needle aspiration cytology of subcutaneous cysticercosis. Diagn Cytopathol 1991;7:223-4. |
| 24. | Goyal P, Sehgal S, Ghosh S, Mittal D, Kumar A, Singh S, et al. A cytological study of palpable superficial nodules of parasitic origin: A study of 41 cases. Pathol Res Int 2014;2014:373472. |
Correspondence Address:
Dr. Gousia Rather
H. No. 83/9, Trikuta Nagar, Near Police Station, Jammu - 180 012, Jammu and Kashmir
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/JOC.JOC_77_17
[Figure 1], [Figure 2]
[Table 1], [Table 2], [Table 3], [Table 4] |
|
| This article has been cited by | | 1 |
Primary subcutaneous hydatidosis of axilla |
|
| Immanuel Pradeep, Seetu Palo, Ashutosh Rath, Jitendra Singh Nigam, Shailaja Prabhala, Nyna Sindhu Akula | | Diagnostic Cytopathology. 2022; | | [Pubmed] | [DOI] | | | 2 |
Optical Coherence Tomography Features of Ocular Cysticercosis: A Review of Literature With Observer Variation |
|
| Abhishek Sheemar, Nripen Gaur, Pratima Singh Thakur, Pallvi Sharma, Brijesh Takkar, Sumeet Khanduja | | Ophthalmic Surgery, Lasers and Imaging Retina. 2022; 53(8): 446 | | [Pubmed] | [DOI] | | | 3 |
Cytomorphological spectrum of cysticercosis: A study of 26 cases |
|
| Deepika Gupta, Prajwala Gupta, Swasti Jain, Seema Rahar | | Cytopathology. 2021; 32(6): 802 | | [Pubmed] | [DOI] | | | 4 |
Detection of Cysticercosis in Palpable Subcutaneous and Intramuscular Nodules in FNAC - A Chance Diagnosis - A Study of 15 Cases |
|
| Anuradha De Pati, Selim Akhtar | | Journal of Evolution of Medical and Dental Sciences. 2020; 9(29): 2049 | | [Pubmed] | [DOI] | |
|
|
|
|
|
|
|
|
|
|
|
|
| Article Access Statistics | | | Viewed | 5157 | | | Printed | 89 | | | Emailed | 0 | | | PDF Downloaded | 235 | | | Comments | [Add] | | | Cited by others | 4 | | |
|
|
