Journal of Cytology
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Year : 2018  |  Volume : 35  |  Issue : 4  |  Page : 199-203

Rehydration of air-dried smears with normal saline: An alternative for conventional wet fixation method in cervical cytological study

Department of Pathology, B.L.D.E University's Shri. B. M. Patil Medical College Hospital and Research Centre, Bijapur, Karnataka, India

Correspondence Address:
Dr. Sachin Shivaji Kapse
Department of Pathology, B.L.D.E University's Shri. B. M. Patil Medical College Hospital and Research Centre, BLDE Campus, Sholapur Road, Vijayapura, Bijapur - 586 103, Karnataka
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/JOC.JOC_186_17

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Context: Papanicolaou (Pap) smear is an effective exfoliative cytological investigation done for early recognition of cervical cancer. It also plays role in diagnosis of inflammatory lesions of cervix. Aims: The aim of this study is (1) to compare the cytomorphological features in conventional Pap smear (C-PAPS) and rehydrated air-dried Pap smear (RADPS) and (2) to evaluate the efficacy of RADPS in cytodiagnosis of cervical lesion by comparing with cytomorphological features of conventional wet-fixed Pap smear. Subjects and Methods: Paired cervical smears were prepared for 247 patients. One was labeled as C-PAPS and another was labeled as RADPS. Comparison of both smears was done for various cytomorphological parameters. Results: Out of 247 smears, 2.4% RADPS and 7.3% C-PAPS were reported as unsatisfactory. Red blood cell (RBC) background was present in 2% of RADPS and 42% of C-PAPS. Cytolysis and air-drying artifact were observed more in C-PAPS amounting to 2% and 4% in RADPS and 11% and 15% in C-PAPS. Cytoplasmic staining (97% RADPS vs. 94% C-PAPS) was superior in RADPS. Cell border, nuclear border, and chromatin of squamous and endocervical cells were better appreciated on RADPS compared to C-PAPS, and also statistically significant difference was observed. Conclusion: Rehydrated air-dried technique can be satisfactory alternative for conventional wet fixation method which can be followed routinely or in conjugation with C-PAPS, especially in cervical screening programs.

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