| ORIGINAL ARTICLE |
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| Year : 2018 | Volume
: 35
| Issue : 3 | Page : 153-158 |
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Role of p16/Ki-67 Dual immunostaining in detection of cervical cancer precursors
Diya Das, Moumita Sengupta, Keya Basu, Mona Tirkey, Chhanda Datta, Uttara Chatterjee
Department of Pathology, Institute of Postgraduate Medical Education and Research, Kolkata, West Bengal, India
Correspondence Address:
Dr. Moumita Sengupta
Department of Pathology, Institute of Post Graduate Medical Education and Research, Kolkata - 700 020, West Bengal
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/JOC.JOC_4_17
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Background: Pap-smears-based cytology and human papilloma virus testing have their own limitations in detecting cervical precancerous lesions, and still need further standardization. Co-expression of p16ink4a and Ki-67 can be used as additional biomarker. Aims: To study the role of liquid-based cytology and the dual immunostaining for p16/Ki-67 in predicting the presence of significant lesion in cases of mild cytological atypia. Materials and Methods: A prospective, cross-sectional study was performed in the Department of Pathology, in collaboration with Department of Obstetrics and Gynecology over 15 months including 545 patients. Immunocytochemistry followed by colposcopy-guided biopsy were performed in 52 cases with epithelial abnormalities. Results: Thirty-five cases (67%) were dual-stain positive among the cases with epithelial abnormalities. In the ASC-US and LSIL group, the sensitivity and specificity of the immunostaining in diagnosing CIN2+ lesions were 100 and 70% and 87.5 and 100%, respectively. p16/Ki-67 positivity also increased with cytological severity which in turn corresponded with histological findings: it reached from 33% in ASC-US to 100% in both HSIL and SCC categories. Conclusion: This dual immunostaining may potentially be a useful tool in the triage of the ASC-US and the LSIL group, considering the high sensitivity and specificity values.
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