Journal of Cytology
Home About us Ahead of print Instructions Submission Subscribe Advertise Contact e-Alerts Login 
Users Online:436
  Print this page  Email this page Small font sizeDefault font sizeIncrease font size

 Table of Contents    
Year : 2012  |  Volume : 29  |  Issue : 1  |  Page : 11-15
Diagnostic utility of the cell block method versus the conventional smear study in pleural fluid cytology

1 Department of Pathology, Hassan Institute of Medical Sciences, Hassan, India
2 Department of Pathology, BLDEA's BM Patil Medical College, Bijapur, India

Click here for correspondence address and email

Date of Web Publication27-Feb-2012


Background: The cytological examinations of serous effusions have been well-accepted, and a positive diagnosis is often considered as a definitive diagnosis. It helps in staging, prognosis and management of the patients in malignancies and also gives information about various inflammatory and non-inflammatory lesions. Diagnostic problems arise in everyday practice to differentiate reactive atypical mesothelial cells and malignant cells by the routine conventional smear (CS) method.
Aims: To compare the morphological features of the CS method with those of the cell block (CB) method and also to assess the utility and sensitivity of the CB method in the cytodiagnosis of pleural effusions.
Materials and Methods: The study was conducted in the cytology section of the Department of Pathology. Sixty pleural fluid samples were subjected to diagnostic evaluation for over a period of 20 months. Along with the conventional smears, cell blocks were prepared by using 10% alcohol-formalin as a fixative agent. Statistical analysis with the 'z test' was performed to identify the cellularity, using the CS and CB methods. Mc. Naemer's χ2 test was used to identify the additional yield for malignancy by the CB method.
Results: Cellularity and additional yield for malignancy was 15% more by the CB method.
Conclusions: The CB method provides high cellularity, better architectural patterns, morphological features and an additional yield of malignant cells, and thereby, increases the sensitivity of the cytodiagnosis when compared with the CS method.

Keywords: Cell block; conventional smear; pleural effusions; sensitivity

How to cite this article:
Shivakumarswamy U, Arakeri SU, Karigowdar MH, Yelikar B R. Diagnostic utility of the cell block method versus the conventional smear study in pleural fluid cytology. J Cytol 2012;29:11-5

How to cite this URL:
Shivakumarswamy U, Arakeri SU, Karigowdar MH, Yelikar B R. Diagnostic utility of the cell block method versus the conventional smear study in pleural fluid cytology. J Cytol [serial online] 2012 [cited 2023 Mar 22];29:11-5. Available from:

   Introduction Top

Cytological examination of serous fluids is one of the commonly performed investigation. The accurate identification of cells as either malignant or reactive mesothelial cells is a diagnostic problem in conventional cytological smears. The cell block (CB) technique is one of the oldest methods for the evaluation of body cavity fluids. [1] However, a new method of cell block preparation by using 10% alcohol-formalin as a fixative was used, to identify the sensitivity of the diagnosis in comparison with the conventional smear (CS) study. The main advantages of the CB technique are preservation of tissue architecture and obtaining multiple sections for special stains and immunohistochemistry. [2]

   Materials and Methods Top

Pleural fluids were collected for cytological evaluation in the cytology section of the Department of Pathology, from September 2005 to April 2007. Ten milliliters of fresh pleural fluid sample was received. It was divided into two equal parts of five milliliters each. One part was subjected to conventional smear cytology and the other part for the cell block technique. Thus, the same sample was evaluated for a comparative study.

Conventional smear technique

The five milliliter sample was centrifuged at 2500 rpm for 15 minutes. A minimum of two thin smears were prepared from the sediment. One smear was prepared after air drying and stained with the May-Grünwald-Giemsa stain. The other smear was immediately fixed in 95% alcohol and stained with the Papanicolaou stain.

Cell block technique

The 5 mL sample that remained was subjected to fixation for one hour by mixing with 5 mL of 10% alcohol-formalin (i.e., nine parts of 90% alcohol and one part of 7.5% formalin). After one hour, this 10 ml fluid was centrifuged at 2500 rpm for 15 minutes. The supernatant was discarded and a further 3 mL of fresh 10% alcohol-formalin was once again added to the sediment and it was kept for one day. On the following day, the sediment containing the cell button of the pleural fluid sample was scooped out on to the filter paper and this cell button sediment sample was processed along with other routine histopathological specimens. The paraffin embedded cell button (cell block) sections of 4-6 m thickness were prepared and stained with the hematoxylin and eosin stain. Special stains like the periodic acid Schiff (PAS) and mucicarmine were performed wherever necessary.

Interpretation of CS versus CB

The samples were studied in detail taking into account the available clinical data, various investigation reports and morphological details. The samples were categorized as benign, suspicious for malignancy, or malignant lesions. The various morphological criteria that were taken into account included the cellularity, arrangement of the cells (acini, papillae and cell balls) and the cytoplasmic and nuclear details. All these criteria were put together and used for classifying the various cytomorphological patterns. A comparative evaluation of CS versus CB technique was conducted. The cytomorphological characters were studied to identify the malignancy and the most probable primary site.

   Results Top

Sixty pleural fluid samples were subjected to the CS and CB method techniques. The age ranged from 18 to 90 years. Maximum samples were from the 51-60 year age group (21%). The least number of samples was from the age group of

11-20 years (2%). Male patient samples (thirty-five) outnumbered the female patient samples. Cellularity was more by the CB method when compared to the CS method. Architectural patterns, such as, glands, sheets, three-dimensional cell clusters and cell balls were commonly observed in the CB method, whereas, singly scattered cells were predominant findings in CS.

After analysis of the above samples, they were categorized as benign, suspicious for malignancy, [Figure 1] or malignant samples [Figure 2]. By the cell block method, an additional nine cases were detected as malignant, that is, 15% more diagnostic yield for malignancy. These samples were reported as either suspicious for malignancy or benign samples. Further analysis showed a discrepancy in 12 cases [Table 1]. In the conventional smears, out of seven reported benign samples, two were reported as florid mesothelial hyperplasia, four samples were misdiagnosed, as the morphology was obscured by a hemorrhagic, dirty background, plenty of inflammatory cells and reactive mesothelial cells. One more sample was misdiagnosed as an inflammatory smear. However, these seven samples were reported as malignant by the CB method. Out of the five samples that were reported as suspicious for malignancy in CS, two samples were diagnosed as malignant effusions and the other three as benign lesions by the CB method.
Figure 1: Photomicrograph showing scattered and clusters of suspicious cells in CS (Giemsa, ×400)

Click here to view
Figure 2: Photomicrograph showing bizarre shaped cells with pleomorphic nuclei in cell block from the same sample shown in Figure 1 (H and E, ×400)

Click here to view
Table 1: Analysis of discrepancies between CS and CB in the pleural fluid

Click here to view

The malignant effusions were more common in females than males. The female-to-male ratio was 3:1 for malignant effusions. The most common primary identified was from the breast. Out of 10 cases of malignant pleural effusions, the primary was known in seven cases, which included three cases of carcinoma breast from female patients and two cases each of carcinoma of the lung (one from a male and another from a female patient) and gastrointestinal tract (one from a male and another from a female patient). In the remaining three cases, the primary could not be detected as the patients were lost to follow-up.

Statistical analysis of these 60 samples showed high cellular yield by the CB method rather than the CS method. For this a z test was done that showed a P value of 0.038. Mc. Naemer's χ 2 test was used for analysing benign and malignant lesions by the CB method and CS methods in which the P value was 0.0021. Results showed 100% sensitivity by the CB method in the diagnosis of malignancy. Therefore, in this study, utility of the CB method in the cytodiagnosis of malignant effusion was highly significant as compared to the conventional smear method.

   Discussion Top

The cytological examination of serous effusions has increasingly gained acceptance in clinical medicine, to such an extent that a positive diagnosis is often considered the definitive test and obviates explorative surgery. It is important not only in the diagnosis of malignant lesions, but also helps in staging and prognosis. [3]

The development of malignant pleural effusion is a common complication of cancers like pulmonary and gastric carcinomas. [4] Examination of fluids from the serous cavities of the body is an essential component of management in adult patients. Malignant neoplasms, especially lymphoid neoplasms, represent a major cause of death in children and in these cases cytological examination is very useful in their management. [5]

One of the most common problems in CS cytology is to distinguish reactive mesothelial cells from metastatic neoplasms. The difficulty is either secondary to marked atypia of mesothelial cells caused by the microbiological, chemical, physical, immunological, or metabolic insults to the serous membranes or to the subtle cytomorphological features of some malignant neoplasms, particularly well-differentiated adenocarcinomas. The problem may become compounded by artefacts from poor fixation, preparation, or staining techniques. [6] Although the preparation of CS is a much simpler procedure than that of paraffin sections, it has limitations, that is, lack of tissue architecture. In some cases, appreciation of tissue architecture make diagnosis easier. [7] Another limitation of the conventional cytological examination of effusions is that it has a sensitivity of only 40-70% for the presence of malignant disease due to overcrowding of cells, cell loss and different laboratory processing methods. Others like reactive mesothelial cells, abundance of inflammatory cells and paucity of representative cells contribute to considerable difficulties in making conclusive diagnosis on conventional smears. [8]

Since the introduction of the CB technique by Bahrenburg nearly a century ago, it has been used routinely for processing fluids. In 1928, Zemansky concluded that the CB method was superior to the CS technique and that examination of materials other than pleural and ascitic fluids was unreliable. Cancer cells in the pleural or ascitic fluid are almost always indicative of metastatic cancer, as tumors arising from mesothelial cells lining these spaces are rare. When present, the tumor cells are usually numerous and frequently clusters may be found. The glandular forms are more reliable on CB. The demonstration of mucin in the tumor cells is evidence that they originate from a glandular epithelium. [9] Diagnostic problems arise whenever there is only marginal morphological distinction, for example, between reactive mesothelial cells and poorly differentiated malignant cells. [10] Earlier methods of CB preparations did not receive much attention, probably due to the lack of standardized technique. In fact the main problem with the CB preparation is the risk of losing material during preparation. Some researchers used agar, plasma/thromboplastin to bind the sedimented cells, but they have some disadvantages. [7],[8]

The advantages of the CB procedure include:

  1. Recognition of histological patterns of diseases that sometimes cannot be identified reliably in conventional smears.
  2. Possible to study multiple sections by routine staining, special staining and immunocytological procedures.
  3. Less cellular dispersal, which permits easier microscopic observation than do traditional smears.
  4. Less difficulty in spite of background showing excess blood on microscopic observation.
  5. Possibility of storing slides for retrospective studies. Storage of the CS is a practical problem. [11]

For this reason, an attempt was made to prepare and analyze both CS and CB from the same specimen. In this study, due consideration was given to age, sex, site of effusion, clinical and radiological findings, to arrive at a final diagnosis and also to identify the primary malignant lesion. Cell blocks may provide diagnostic information complementary or additional to that obtained from an examination of the cell smears. However, morphological preservation is often unsatisfactory in cell blocks processed by routine schedules used for surgical specimens. The 7.5% buffered formalin is the optimal formalin solution and a shortened time through xylene is desirable. Nevertheless, xylene has a marked shrinking effect on cells. If the usefulness of a cell block is to be maximized, fixation and processing of the samples has to be modified so that the morphology approaches that of the conventional paraffin sections of the surgical specimens. [7] In our study, we used 10% alcohol-formalin as a fixative for the CB preparation. By doing this, we got better cellularity as formalin minimized the cell loss by forming protein cross links and gel formation that could not be dissolved by various chemicals used for processing.

Apart from increased cellularity, better morphological details were also obtained with CB, which included, preservation of the architectural patterns such as, cell balls, papillae and three-dimensional clusters, better nuclear and cytoplasmic preservation, intact cell membrane and crisp chromatin details [Figure 3]. All these features increased the sensitivity of the diagnosis of malignancies by the CB methods, which were reported as negative in CS.
Figure 3: Photomicrograph showing acini, papillae and pleomorphic cells in CB (H and E, ×400)

Click here to view

Reactive mesothelial cells have in the past been responsible for simulating malignancy in CS, largely due to the formation of rosettes, pseudoacini, or acini, with or without the presence of prominent nucleoli. The CB effectively puts both the features in their proper prospective: That is, the nucleoli do not appear as prominent as in the CS, and the pseudoacinar or acinar structures can be better appreciated when present, in the CB. Similar findings were noticed in the Dekker and Bupp study. [3] More important still, this CB is a valuable tool in the evaluation of well-differentiated adenocarcinomas such as tumors of the breast, lung, or gastrointestinal tract. These tumors have few malignant characters in CS, while the presence of true acini is seen in the CB, together with mucin, when stained for mucin, and are indicative of malignancy. The other advantage of CB is concentration of cellular material in one small area that can be evaluated at a glance with all cells lying in the same focal plane of the microscope. It bridges the gap between cytology and histology. [3]

Comparison of the cytodiagnosis of malignant effusions in the present study been compared with other studies. An additional yield for malignancy is similar to the results of the Dekkar and Bupp [3] study. They reported that samples obtained by the combined CB and CS techniques for malignant lesions were double that of the CS technique only.

The present study results for primary lesions were correlating with the Sears and Hajdu [4] and Johnston [12] studies.Sears and Hajdu [4] reported that the most common primary neoplasms causing pleural effusions were carcinoma of the breast (24%), followed by lung (19%) and lymphoreticular system (16%), and in 15% of the cases the primary site was unknown. In our study for pleural fluid analysis, carcinoma of the breast (30%) was the most common primary followed by primary in the lung (20%) and gastrointestinal tract (20%) and in 30% of the cases the primary site was unknown. Most of the tumors were of the adenocarcinoma type. In the present study, out of seven unknown primary cases, one case was suspected to be from papillary carcinoma of the thyroid, as the morphological features showed the presence of papillae, optically clear nuclei and psammoma bodies. In another case carcinoid tumor was suspected due to the presence of morphological features and clinical manifestations such as headache and flushing. The 5-HT assay was advised, but the patient was lost to follow-up. In another case, the radiological features revealed findings of pancreatic carcinoma, but this case was also lost to follow-up. In these three suspected cases, retrospective clinical examination and other investigations did not show primary in the lung, breast, gastrointestinal tract, or genitourinary sites. In the remaining four unknown cases, the primary was not known, as the clinical details were not available and these patients also were lost to follow up.

We noted the presence of pericellular lacunae in more than 60% of the cases of adenocarcinoma, especially of the mucin secreting type, characterized by large cell clusters. [6] Bull's eye (Target) inclusion - like findings were seen in one case of metastatic adenocarcinoma of the gastrointestinal tract, which was an additional finding in the diagnosis of malignant effusions. [13] Hence, the sections from CB provided additional information for a definite diagnosis, as it allowed recovery of minute cellular material and was valuable for histochemical and immunohistochemical methods. [14],[15]

To conclude, our present study results showed that the cellblock technique, by using 10% alcohol-formalin as a fixative, was a simple, inexpensive method, and did not require any special training or instrument. The CB method yielded more cellularity and better architectural patterns which improved the diagnosis of malignancy by 15%. Multiple sections could be obtained if required for special stain or an Immunohistochemistry (IHC) study. Therefore, the CB technique could be considered as a useful adjuvant in evaluating fluid cytology for a final cytodiagnosis, along with the routine CS method. [16]

   References Top

1.Wojcik EM, Selvagi SM. Comparison of smears and s in the fine needle aspiration diagnosis of recurrent gynecologic malignancies. Acta Cytol 1991;35:773-6.  Back to cited text no. 1
2.Nathan NA, Narayan E, Smith MM, Horn MJ. Cytology-improved preparation and its efficacy in diagnostic cytology. Am J Clin Pathol 2000;114:599-606.  Back to cited text no. 2
3.Dekker A, Bupp PA. Cytology of serous effusions. An investigation into the usefulness of cell blocks versus smears. Am J Clin Pathol 1978;70:855-60.  Back to cited text no. 3
4.Sears D, Hajdu SI. The cytologic diagnosis of malignant neoplasms in pleural and peritoneal effusions. Acta Cytol 1987;31:85-97.  Back to cited text no. 4
5.Wong JW, Pitlik D, Abdul-Karim FW. Cytology of pleural, peritoneal and pericardial fluids in children: A 40 years summary. Acta Cytol 1997;41:467-73.  Back to cited text no. 5
6.Price BA, Ehya H, Lee JH. Significance of pericellular lacunae in cell blocks of effusions. Acta Cytol 1992;36:333-7.  Back to cited text no. 6
7.Kung IT, Yuen RW, Chan JK. Technical notes. Optimal formalin fixation and processing schedule of cell blocks from the fine needle aspirates. Pathology 1989;21:143-5.  Back to cited text no. 7
8.Mezger J, Stotzer O, Schilli G, Bauer S, Wilmanns W. Identification of carcinoma cells in ascitic and pleural fluid. Comparison of four panepithelial antigens with carcinoembryonic antigen. Acta Cytol 1992;36:75-81.  Back to cited text no. 8
9.Velios F, Griffin J. Examination of body fluids for tumour cells. Am J Clin Pathol 1954;24:676-81.  Back to cited text no. 9
10.Bousfield LR, Greenberg ML, Pacey F. Cytogenetic diagnosis of cancer from body fluids. Acta Cytol 1985;29:768-73.  Back to cited text no. 10
11.Zito FA, Gadaleta CD, Salvatore C, Filático R, Labriola A, Marzullo A, et al. A modified cell block technique for fine needle aspiration cytology. Acta Cytol 1995;39:93-9.  Back to cited text no. 11
12.Johnston WW. The malignant pleural effusion: A review of cytopathologic diagnosis of 584 specimens from 472 consecutive patients. Cancer 1985;56:905-9.  Back to cited text no. 12
13.Kumar PV, Eqbali S, Monabati A, Talei AR. Bull's eye (Target) inclusions in neoplastic cells in malignant serous effusions: A study of 289 cases. Acta Cytol 2000;44:543-6.  Back to cited text no. 13
14.Leung SW, Bedard YC. Methods in Pathology: Simple mini block technique for cytology. Mod Pathol 1993;6:630-2.  Back to cited text no. 14
15.Kushwaha R, Shashikala P, Hiremath S, Basavaraj HG. Cells in pleural fluid and their value in differential diagnosis. J Cytol 2008;25:138-43.  Back to cited text no. 15
  Medknow Journal  
16.Thapar M, Mishra RK, Sharma A, Goyal V, Goyal V. Critical analysis of cell block versus smear examination in effusions. J Cytol 2009;26:60-4.  Back to cited text no. 16
[PUBMED]  Medknow Journal  

Correspondence Address:
Udasimath Shivakumarswamy
Department of Pathology, Hassan Institute of Medical Sciences (HIMS), Hassan 573 201, Karnataka
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0970-9371.93210

Rights and Permissions


  [Figure 1], [Figure 2], [Figure 3]

  [Table 1]

This article has been cited by
1 DNA image cytometry ploidy analysis technique improves the detection rate of pleural effusion cytology
Zhenhua Ma, Pan Li, Xiaodong Gai, Xingang Li, Bo Sun, Taisheng Wang, Pingping Jiang, Haitao Wang, Jihong Zhang
Diagnostic Cytopathology. 2022;
[Pubmed] | [DOI]
2 Diagnostic sensitivity of pleural fluid cytology in malignant pleural effusions: systematic review and meta-analysis
Shayan Kassirian, Stephanie N Hinton, Sean Cuninghame, Rushil Chaudhary, Alla Iansavitchene, Kayvan Amjadi, Inderdeep Dhaliwal, Cady Zeman-Pocrnich, Michael A Mitchell
Thorax. 2022; : thoraxjnl-
[Pubmed] | [DOI]
Sobia Afreen, Idrees Akhtar Afroze
[Pubmed] | [DOI]
4 Comparative evaluation of diagnostic efficacy of cell block versus aspiration cytology
Nikhil Chaudhary, Subhashish Das, Snigdha Sinha
Advances in Human Biology. 2022; 0(0): 0
[Pubmed] | [DOI]
5 Diagnosis of pleural fluid effusions by cell block and pleural biopsy – A comparative study
SS Sabitha Rani, ImmadiSudhakar Vamshidhar, NitinAshok John, Jyoti John
Journal of Cytology. 2022; 0(0): 0
[Pubmed] | [DOI]
Sabari Devi, Rituparna Dhekial
[Pubmed] | [DOI]
7 Epithelial Membrane Antigen, Vimentin, Desmin, Calretinin, E-Cadherin on Cell Block Preparations to Distinguish Well Differentiated Adenocarcinoma from Benign, Reactive, Atypical Mesothelial Cells
Neha Jaiswal, Jayant Makrande, Sunita Vagha
Journal of Evolution of Medical and Dental Sciences. 2021; 10(18): 1302
[Pubmed] | [DOI]
8 ‘Rollover’ abdominal paracentesis versus standard technique: protocol of a crossover randomized comparative trial
Vishal Sharma, Daya Krishna Jha, Manish Rohilla, Chandan K Das, Harjeet Singh, Santhosh Irrinki, Aashima Arora, Subhas C Saha, Pankaj Gupta, Harshal S Mandavdhare, Usha Dutta, Aman Sharma
Future Oncology. 2021; 17(26): 3425
[Pubmed] | [DOI]
9 Architectural aspects of cell-blocks as small biopsies
Swati Satturwar, Liron Pantanowitz
Cytojournal. 2021; 18: 5
[Pubmed] | [DOI]
10 Comparison of Vascular Endothelial Growth Factor-A (VEGF-A) Level in Pleural Fluid of Patients with Malignant and Nonmalignant Pleural Effusion
Wahyuni Hariyanto, Laksmi Wulandari, Isnu Pradjoko, Gatot Soegiarto
Indian Journal of Surgery. 2021; 83(1): 132
[Pubmed] | [DOI]
11 Cell block examination of pleural fluid, an underused and overlooked method for evaluation of malignant pleural effusion
Biplab K. Saha, Alyssa Bonnier
The American Journal of the Medical Sciences. 2021;
[Pubmed] | [DOI]
12 Immunohistochemical analysis using cell block technique leads accurate diagnosis of ovarian malignant lymphoma: A case report
Kosuke Hiramatsu, Kaoru Fukui, Ikuko Sawada, Kentaro Kuritani, Masafumi Takahashi, Tomoko Kanayama, Hiromi Ugaki, Mirang Kim, Megumu Inoue, Hayato Kimura, Kyoka Amemiya
International Journal of Surgery Case Reports. 2020; 69: 1
[Pubmed] | [DOI]
13 High Efficient Isolation of Tumor Cells by a Three Dimensional Scaffold Chip for Diagnosis of Malignant Effusions
Shan Tian, Shi-Bo Cheng, Ying-Yun Guo, Min Xie, Na Zhan, Zhi Zeng, Wei-Hua Huang, Wei-Guo Dong
ACS Applied Bio Materials. 2020; 3(4): 2177
[Pubmed] | [DOI]
14 Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm Function
Violetta Borelli, Monica Martinelli, Stefania Luppi, Francesca Vita, Federico Romano, Francesco Fanfani, Elisa Trevisan, Fulvio Celsi, Giuliano Zabucchi, Fabrizio Zanconati, Cristina Bottin, Giuseppe Ricci
Frontiers in Physiology. 2020; 10
[Pubmed] | [DOI]
15 Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in “Selected” Diagnostic Practice
Paola Parente, Claudia Covelli, Magda Zanelli, Domenico Trombetta, Illuminato Carosi, Cristiano Carbonelli, Marco Sperandeo, Luca Mastracci, Giovanni Biancofiore, Maurizio Zizzo, Marco Taurchini, Stefano Ascani, Paolo Graziano
Diagnostics. 2020; 10(10): 748
[Pubmed] | [DOI]
R. P. Siddiqui, Mohd. Jafar Memon, Shraddha Sahu
[Pubmed] | [DOI]
17 The diagnostic utility of cell block in fine needle aspiration cytology of palpable breast lesions in a Nigerian tertiary health institution
Fred John Nnaemeka Obiajulu, Adetola Olubunmi Daramola, Charles Chidozie Anunobi, Nzechukwu Zimudo Ikeri, Fatimah Biade Abdulkareem, Adekunbiola Aina Banjo
Diagnostic Cytopathology. 2020; 48(12): 1300
[Pubmed] | [DOI]
Ankan Nandi, Debarshi Jana
[Pubmed] | [DOI]
19 Challenges of pleural aspirate cytology: A 5-year review
Ikponmwosa Obahiagbon, MojisolaOlutayo Udoh
Annals of Tropical Pathology. 2020; 11(1): 33
[Pubmed] | [DOI]
20 Evaluation of role of cell block with immunohistochemistry in differentiating hepatocellular carcinoma from metastatic adenocarcinoma
PalashKumar Mandal, Soumi Pradhan, Anindya Adhikari, Mimi Gangopadhyay, DuttaPal Rupsha, Subrata Bhattacharya
CHRISMED Journal of Health and Research. 2020; 7(2): 95
[Pubmed] | [DOI]
21 Evaluation of cell blocks from effusion specimens in Gynecologic Oncopathology: An experience of 220 cases, diagnosed at a Tertiary Cancer Referral Center
Bharat Rekhi, Srushti Karmarkar, Chhavi Gupta, KedarK Deodhar, Santosh Menon, Saleem Pathuthara, Amita Maheshwari, TS Shylasree, Sudeep Gupta
Indian Journal of Pathology and Microbiology. 2020; 63(3): 427
[Pubmed] | [DOI]
22 Processing and reporting of cytology specimens from mediastinal lymph nodes collected using endobronchial ultrasound-guided transbronchial needle aspiration: a state-of-the-art review
InderpaulSingh Sehgal, Nalini Gupta, Sahajal Dhooria, AshutoshNath Aggarwal, Karan Madan, Deepali Jain, Parikshaa Gupta, NehaKawatra Madan, Arvind Rajwanshi, Ritesh Agarwal
Journal of Cytology. 2020; 37(2): 72
[Pubmed] | [DOI]
Ravi Singh, Sukhwant Singh Hayer, Vaibhav Pandurang Mane, Rupali Gopal Yelave, Vishrabdha Rahul Pawar
Journal of Evolution of Medical and Dental Sciences. 2018; 7(06): 771
[Pubmed] | [DOI]
24 Differential diagnosis of mesenchymal neoplasms of the digestive tract by cell block and immunohistochemistry
César Vivian Lopes,Péttala Rigon,Cláudio Galleano Zettler,Antônio Atalíbio Hartmann
Cytopathology. 2018; 29(6): 531
[Pubmed] | [DOI]
25 Targeted deep sequencing of effusion cytology samples is feasible, informs spatiotemporal tumor evolution, and has clinical and diagnostic utility
Jonas Leichsenring,Anna-Lena Volckmar,Martina Kirchner,Daniel Kazdal,Mark Kriegsmann,Fabian Stögbauer,Teresa Bockmayr,Frederick Klauschen,Felix J.F. Herth,Roland Penzel,Arne Warth,Peter Schirmacher,Volker Endris,Albrecht Stenzinger
Genes, Chromosomes and Cancer. 2018; 57(2): 70
[Pubmed] | [DOI]
26 Metastatic hepatocellular carcinoma masquerading as soft tissue sarcoma: A diagnostic challenge
Debasis Gochhait,Priyadarshini Dehuri,Vidhyalakshmi Rangarajan,GangaBhavani Kundeti,Bhawana Badhe,Ramkumar Govindarajalou
Diagnostic Cytopathology. 2018; 46(6): 553
[Pubmed] | [DOI]
27 Diagnosis of anaplastic lymphoma kinase rearrangement in cytological samples through a fluorescence in situ hybridization-based assay: Cytological smears versus cell blocks
Federica Zito Marino,Giulio Rossi,Matteo Brunelli,Maria Gabriella Malzone,Giuseppina Liguori,Giuseppe Bogina,Alessandro Morabito,Gaetano Rocco,Renato Franco,Gerardo Botti
Cancer Cytopathology. 2017; 125(5): 303
[Pubmed] | [DOI]
28 Examination of cytological smears and cell blocks of pleural fluid: Complementary diagnostic value for malignant effusions
J.M. Porcel,M. Quirós,S. Gatius,S. Bielsa
Revista Clínica Española. 2017; 217(3): 144
[Pubmed] | [DOI]
29 Examination of cytological smears and cell blocks of pleural fluid: Complementary diagnostic value for malignant effusions
J.M. Porcel,M. Quirós,S. Gatius,S. Bielsa
Revista Clínica Española (English Edition). 2017; 217(3): 144
[Pubmed] | [DOI]
30 Cell tube block: a new technique to produce cell blocks from fluid cytology samples
Ricardo Marcos,Marta Santos,Carla Marrinhas,Mario Caniatti
Veterinary Clinical Pathology. 2017; 46(1): 195
[Pubmed] | [DOI]
Gopinath Barui, Manas Talukdar, Malay Sarkar
Journal of Evidence Based Medicine and Healthcare. 2017; 4(94): 5754
[Pubmed] | [DOI]
Megha Sharma, Kuldeep Singh
Journal of Evidence Based Medicine and Healthcare. 2017; 4(39): 2347
[Pubmed] | [DOI]
33 Diagnostic efficacy of cell block method for vitreoretinal lymphoma
Satoru Kase,Kenichi Namba,Daiju Iwata,Kazuomi Mizuuchi,Nobuyoshi Kitaichi,Yoshiaki Tagawa,Hiromi Okada-Kanno,Yoshihiro Matsuno,Susumu Ishida
Diagnostic Pathology. 2016; 11(1)
[Pubmed] | [DOI]
34 Diagnostic Utility of Pleural Fluid Cell Block versus Pleural Biopsy Collected by Flex-Rigid Pleuroscopy for Malignant Pleural Disease: A Single Center Retrospective Analysis
Shion Miyoshi,Shinji Sasada,Takehiro Izumo,Yuji Matsumoto,Takaaki Tsuchida,Hyun-Sung Lee
PLOS ONE. 2016; 11(11): e0167186
[Pubmed] | [DOI]
35 Increasing diagnostic effectiveness of thyroid nodule evaluation by implementation of cell block preparation in routine US-FNA analysis
Ana Patrícia de Cristo,Heloísa Folgierini Goldstein,Carlo Sasso Faccin,Ana Luiza Maia,Marcia Silveira Graudenz
Archives of Endocrinology and Metabolism. 2016; 60(4): 367
[Pubmed] | [DOI]
36 Role of Cell Block in Diagnostics-A New Paradigm in Cancer Diagnosis
Radha Sharma
International Clinical Pathology Journal. 2015; 1(5)
[Pubmed] | [DOI]
37 MRT Letter: 3D culture of isolated cells: A fast and efficient method for optimizing their histochemical and immunocytochemical analyses
Angiola Berenzi,Nathalie Steimberg,Jennifer Boniotti,Giovanna Mazzoleni
Microscopy Research and Technique. 2015; 78(4): 249
[Pubmed] | [DOI]
Jyotsna Suri, Vivek Gandotra, Deepak Abrol, Subhash Bhardwaj
Journal of Evidence Based Medicine and Healthcare. 2015; 2(39): 6464
[Pubmed] | [DOI]
39 Importance of the Cell Block Technique in Diagnosing Patients with Non-Small Cell Carcinoma Accompanied by Pleural Effusion
Ceyhan Ugurluoglu,Ercan Kurtipek,Yasar Unlu,Hidir Esme,Nuri Duzgun
Asian Pacific Journal of Cancer Prevention. 2015; 16(7): 3057
[Pubmed] | [DOI]
40 Improved endoscopic retrograde cholangiopancreatography brush increases diagnostic yield of malignant biliary strictures
Frederick K Shieh
World Journal of Gastrointestinal Endoscopy. 2014; 6(7): 312
[Pubmed] | [DOI]
41 Implementation of routine thromboplastin-plasma cell block technique in the evaluation of non-gynecologic specimens: A methodologic comparison with conventional cytology
Diana Castro-Villabón,Yubelly Avello,Nathalie Ruiz,Paula A. Rodríguez-Urrego
Journal of Microscopy and Ultrastructure. 2014;
[Pubmed] | [DOI]
42 Citoinclusão de capa leucocitária e medula óssea de cães: padronização da técnica
M.B. Paiva,G.M. Gouveia,H. Fioravanti,M.C.R. Luvizotto
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2014; 66(2): 439
[Pubmed] | [DOI]
43 Preparation of Cell Blocks for Lung Cancer Diagnosis and Prediction: Protocol and Experience of a High-Volume Center
Claudia A. Kossakowski, Alicia Morresi-Hauf, Philipp A. Schnabel, Ralf Eberhardt, Felix J.F. Herth, Arne Warth
Respiration. 2014; 87(5): 432
[Pubmed] | [DOI]
Gayathri M N, Kunal Puri, Satish M K, Ravikumar T, Bharathi M
Journal of Evidence Based Medicine and Healthcare. 2014; 1(9): 1240
[Pubmed] | [DOI]
45 Emprego do cell block de agarose como método complementar no diagnóstico citológico de tumores mamários caninos
Diogo Sousa Zanoni,Silvia Regina Kleeb,José Guilherme Xavier
Ciência Rural. 2013; 43(3): 489
[Pubmed] | [DOI]
46 Cell tube: a new technique for making cell blocks from needle rinses
Ricardo Marcos
Veterinary Clinical Pathology. 2013; 42(4): 401
[Pubmed] | [DOI]
47 Employment of cell block of agarose as additional method in the cytological diagnosis of canine mammary tumors [Emprego do cell block de agarose como método complementar no diagnóstico citológico de tumores mamários caninos]
Zanoni, D.S. and Kleeb, S.R. and Xavier, J.G.
Ciencia Rural. 2013; 43(3): 489-495
48 PAX8 is a novel marker for differentiating between various types of tumor, particularly ovarian epithelial carcinomas (review)
Li, X. and Beihua, K.
Oncology Letters. 2013; 5(3): 735-738
49 the cell block method increases the diagnostic yield in exudative pleural effusions accompanying lung cancer [hücre bloǧu yöntemi akciǧer kanserine eşlik eden eksudatif plevral sivilarda taniyi artirmaktadir]
köksal, d. and demiraǧ, f. and bayiz, h. and koyuncu, a. and mutluay, n. and berktaş, b. and berkoǧlu, m.
turk patoloji dergisi/turkish journal of pathology. 2013; 29(3): 165-170
50 cell tube: a new technique for making cell blocks from needle rinses
marcos, r.
veterinary clinical pathology. 2013; 42(4): 401-402
51 PAX8 is a novel marker for differentiating between various types of tumor, particularly ovarian epithelial carcinomas
Oncology Letters. 2013; 5(3): 735
[Pubmed] | [DOI]


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Email Alert *
    Add to My List *
* Registration required (free)  

    Materials and Me...
    Article Figures
    Article Tables

 Article Access Statistics
    PDF Downloaded1241    
    Comments [Add]    
    Cited by others 51    

Recommend this journal