| ORIGINAL ARTICLE |
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| Year : 2011 | Volume
: 28
| Issue : 4 | Page : 159-164 |
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Defining the validity of classical and non-classical cellular changes indicative of low-grade squamous intraepithelial lesion encompassing human papillomavirus infection in relation to human papillomavirus deoxyribonucleic acid testing
Veena Kashyap1, Suresh Hedau2, Suresh Bhambhani1
1 Department of Cytology, Institute of Cytology and Preventive Oncology (DHR), Noida, UP, India
2 Department of Molecular Oncology, Institute of Cytology and Preventive Oncology (DHR), Noida, UP, India
Correspondence Address:
Veena Kashyap
Department of Cytology, Institute of Cytology and Preventive Oncology (DHR), Plot I-7, Sector-39, Noida, UP
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0970-9371.86340
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Background: Human papillomavirus (HPV) infection as of now has been beyond doubt to be the causative agent for cervical carcinoma. Its morphological identification in Pap smear is important.
Aim: To define the validity of classical and non-classical cellular changes indicative of low-grade squamous intraepithelial lesion (SIL) encompassing HPV infection in relation to positivity for 'high risk' HPV16 as well as for 'low risk' HPV6/11.
Materials and Methods : A total of 3000 Papanicolaou smears were screened, of which 150 were reported as low grade-SIL encompassing HPV infection (LSIL-HPV). Subsequently cervical scrapes from these 150 subjects, along with equal number of normal women as controls, were collected and processed for HPV deoxy-ribonucleic acid testing by polymerase chain reaction (PCR).
Results: On the basis of cytomorphological characteristics in Pap smears, HPV infection were categorized into the following two groups: Classical (koilocytic) changes (CC) encountered in 30 women and non-classical changes (NCC) encountered in 120 women. It was observed that 21 (70%) CC and 46 (38.3%) NCC of HPV infection were positive for HR-HPV16; however only 12 cases (10%) of NCC and two cases (6.6%) of CC were positive for LR-HPV 6/11. Majority (41.7%) of HPV positive cases were reported in the age group of 25 to 30 years and HPV positivity decreased with the increasing age.
Conclusion: Classical cellular changes are not the only diagnostic features for HPV infection in Pap smear, non-classical diagnostic features also support the diagnosis of HPV infection and may be positive for HR-HPV16. |
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