Journal of Cytology
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Year : 1999  |  Volume : 16  |  Issue : 1  |  Page : 7-14

Statistical significance and interobserver variability of AgNOR Counts in fine needle aspirate smears of thyroid lesions

1 Research Associate, Department of Pathology, King George's Medical College, Lucknow - 226 003, India
2 Associate Professor, Department of Pathology, King George's Medical College, Lucknow - 226 003, India

Correspondence Address:
M M Goel
Department of Pathology, King George's Medical College, Lucknow - 226 003. M.P
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Source of Support: None, Conflict of Interest: None

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The object of the present study is to determine the AgNOR quantitation in fine needle aspirates of thyroid lesions by two different observers and to analyze the interobserver variability by using Bland Altman plot. Forty five cases were taken for this study including 5 cases of thyroiditis, 6 cases of colloid goitre, 15 cases of follicular adenoma, 4 cases of follicular carcinoma, 6cases of papillary carcinoma and 9 cases of anaplastic carcinoma. These smears were stained by one step silver staining colloidal technique and AgNOR dots were counted by two different methods i.e. m AgNOR and p AgNOR by two different observers independently. Malignant lesions had higher number of AgNOR counts in comparison to the goitres and neoplasm. Statistical analysis was done to observe the correlation coefficient and interobserver variability. The correlation coefficient for mAgNOR was 0.78 andforpAgNOR was 0.96. The correlation coefficient between difference and average (r=0.22) indicate that there was an increasing average with difference between the two observers. Our findings also indicate a negative correlation between the difference and the average of p AgNOR counts (r=0.06). There was no statistical evidence of variability between the two observers. Although observers counts do not differ significantly still no cut o ff point could be established to differentiate thyroid lesions.

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